Chemical Composition and Insecticidal Activity of Extracts from Periploca sepium Leaves

Through preliminary systematic test on chemical composition of Periploca sepium leaves,it was found that the leaves containsed phenols,organic acids,saponins,flavonoids and its glycosides,steroids,and triterpenoids.Meanwhile,insecticidal activities of the extracts from P.sepium leaves against Mythimn separate and Macrosiphoniella sanborni were measured by bioactivity-guided sepearation.The results showed that ethyl acetate fraction was more active than others.After ethyl acetate fraction was epearted in the silica gel column,fraction No.3 exhibited the strongest contact toxicity with LC50 2.903 2 mg·mL-1 in 72 hours against M.separate,and 0.526 3 mg·mL-1 in 24 hours against M.sanborni.