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[Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC].

Wen-shan Jin, Yu-yuan Tan, Yougen Chen, Yan Wang

Open publisher page 6 citations

Abstract

OBJECTIVE: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. METHOD: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm. RESULT: The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein. CONCLUSION: This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.

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What this paper is about

OBJECTIVE: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. METHOD: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm. RESULT: The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein. CONCLUSION: This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.

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Available abstract

OBJECTIVE: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. METHOD: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm. RESULT: The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein. CONCLUSION: This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.

Key concepts: Puerarin, Daidzin, Pueraria, Daidzein, Lobata, Chromatography, Chemistry, High-performance liquid chromatography

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[Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC]. — Research Paper | ScholarLens