ScholarLens Back to search
2012•Zhongguo haiyang yaowuRequires access

Protective Effects of Astaxanthin on Hippocampal Neurons Damage Induced by hydrogen peroxide

Shanshan Wang

Open publisher page 1 citations

Abstract

Objective The aim of this study was to investigate the effects of astaxanthin on oxidative damage of hippocampal neurons injured by H2O2.Methods The hippocampal neurons isolated from SD rats and cultured by the B27 serun-free culture medium were subjected to oxidative stress induced by 2.0 10-4mol L-1 H2O2 for 24hr and treated with or without astaxanthin.The cell viability was examined using the microculture tetrazolium test(MTT) assay.The release of LDH,the activity of SOD、CAT、GSH-Px and the content of MDA were determined employing chemical colorimetry.Results Astaxanthin could enhance the survival rate,inhibit the increasing release of LDH of hippocanmal neurons induced by H2O2.Astaxanthin significantly increased the activity of SOD、CAT and GSH-Px(P0.01).Conclusion Astaxanthin has a protective effect on hippocampal neurons against H2O2 damage across wide range of concentrations(10-7～10-5 mol·L-1).

About this research paper

What this paper is about

Objective The aim of this study was to investigate the effects of astaxanthin on oxidative damage of hippocampal neurons injured by H2O2.Methods The hippocampal neurons isolated from SD rats and cultured by the B27 serun-free culture medium were subjected to oxidative stress induced by 2.0 10-4mol L-1 H2O2 for 24hr and treated with or without astaxanthin.The cell viability was examined using the microculture tetrazolium test(MTT) assay.The release of LDH,the activity of SOD、CAT、GSH-Px and the content of MDA were determined employing chemical colorimetry.Results Astaxanthin could enhance the survival rate,inhibit the increasing release of LDH of hippocanmal neurons induced by H2O2.Astaxanthin significantly increased the activity of SOD、CAT and GSH-Px(P0.01).Conclusion Astaxanthin has a protective effect on hippocampal neurons against H2O2 damage across wide range of concentrations(10-7～10-5 mol·L-1).

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective The aim of this study was to investigate the effects of astaxanthin on oxidative damage of hippocampal neurons injured by H2O2.Methods The hippocampal neurons isolated from SD rats and cultured by the B27 serun-free culture medium were subjected to oxidative stress induced by 2.0 10-4mol L-1 H2O2 for 24hr and treated with or without astaxanthin.The cell viability was examined using the microculture tetrazolium test(MTT) assay.The release of LDH,the activity of SOD、CAT、GSH-Px and the content of MDA were determined employing chemical colorimetry.Results Astaxanthin could enhance the survival rate,inhibit the increasing release of LDH of hippocanmal neurons induced by H2O2.Astaxanthin significantly increased the activity of SOD、CAT and GSH-Px(P0.01).Conclusion Astaxanthin has a protective effect on hippocampal neurons against H2O2 damage across wide range of concentrations(10-7～10-5 mol·L-1).

Key concepts: Astaxanthin, Hippocampal formation, Hydrogen peroxide, Oxidative stress, Chemistry, Glutathione, Biochemistry, Pharmacology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Protective Effects of Astaxanthin on Hippocampal Neurons Damage Induced by hydrogen peroxide — Research Paper | ScholarLens