Progresses on cofactor regeneration

Cofactor-dependent enzymes catalyze many useful reactions.Due to the high cost of the cofactors,in situ cofactor regeneration is required for preparative applications. After two decades of research,various cofactors ,such as NAD(P)H,ATP,sugar nucleotides can now be regenerated using enzymatic,chemical, electrochemical, photochemical or genetic engineering based methods. The recent advances in cofactors regeneration are reviewed in this paper.