Evaluation of adenosine preconditioning protective effect on dogs with real-time myocardial contrast echocardiography

Objective To evaluate myocardial systolic function and myocardial blood flow in risk area after adenosine preconditioning(AP) on dogs ischemia reperfusion heart model with real-time myocardial contrast echocardiography(rt-MCE).Methods Thirteen dogs were divided into two groups: AP group(n=7,intravenous infusion of adenosine before ischemia,0.3 mg/min,30 ml) and control group(n=6,intravenous infusion of physiological saline before ischemia,30 ml).The left anterior descending coronary artery was occluded to produce myocardial ischemia reperfusion models(ischemia 3 h,reperfusion 1 h).Contrast agent SonoVue was injected via femoral vein at different time points(baseline before occlusion,ischemia 3 h and reperfusion 1 h),and then short axis images of left ventricular papillary muscle were collected at each time point,and pressure curve of left ventricle was simultaneously recorded.Results Left ventricular systolic pressure(LVSP) in AP group at reperfusion 1 h was higher than that in control group(P0.05).At ischemia 3 h and reperfusion 1 h,the maximal rising rate of left ventricular pressure(+dp/dtmax) was higher than that in control group(both P0.05).With reperfusion,myocardial blood flow in necrosis area and risk area were lower than the normal area,myocardial blood flow in the necrosis area was the lowest(all P0.05).The blood flow in myocardial risk area increased in AP group(P0.05).Conclusion Adenosine preconditioning can be used to improve myocardial systolic function,increase myocardial perfusion in risk area and improve myocardial microcirculation effectively after ischemia reperfusion.