Extraction of doxycycline hydrochloride using anhydrous alcohol and acetone binary aqueous two phase system

Based on the molecular alcohol aqueous two-phase system and the small molecular organic two-phase system,the new method of using anhydrous alcohol and small molecular organic(acetone) with(NH4)2SO4 binary aqueous two phase system for extracting doxycycline hydrochloride was set up.The influence factors on partition behaviors of doxycycline hydrochloride were studied,including the concentration of(NH4)2SO4,anhydrous alcohol and acetone consumption,pH value,temperature and doxycycline hydrochloride.The results showed that when(NH4)2SO4 concentration of 41%,pH value of 4.5～5.0,and the temperature of 25 ℃,mass concentration of doxycycline hydrochloride 70 mg/L,the extraction rate up to 93.61% and 83.081,respectively.