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2011•Journal of Chinese OncologyRequires access

The Growth Inhibition Effect of S100P Gene Silencing by SiRNA on Pancreatic Cancer Cell Line SW1990

Wu-Hua Ni

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Abstract

[Purpose] To investigate the growth inhibition effect of S100P gene silencing by siRNA on human pancreatic cancer cell line SW1990.[Methods] The S100P gene silencing by siRNA was chemically synthesized and transfected into SW1990 cells.The cells were collected at 48h later,and the total RNA and protein was extracted.The mRNA and protein expression of S100P were detected by real-time quantitative PCR and Western blot.The cell growth curve was analyzed by MTT assay.[Results] After transfected with siRNA,S100P mRNA and protein of SW1990 cells were significantly decreased,with inhibition rate of 77% and 74% respectively.The cell growth was significantly inhibited.[Conclusion] S100P gene silencing by siRNA can reduce the expression of S100P in pancreatic cancer cell line SW1990,and can inhibit the cell growth.

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What this paper is about

[Purpose] To investigate the growth inhibition effect of S100P gene silencing by siRNA on human pancreatic cancer cell line SW1990.[Methods] The S100P gene silencing by siRNA was chemically synthesized and transfected into SW1990 cells.The cells were collected at 48h later,and the total RNA and protein was extracted.The mRNA and protein expression of S100P were detected by real-time quantitative PCR and Western blot.The cell growth curve was analyzed by MTT assay.[Results] After transfected with siRNA,S100P mRNA and protein of SW1990 cells were significantly decreased,with inhibition rate of 77% and 74% respectively.The cell growth was significantly inhibited.[Conclusion] S100P gene silencing by siRNA can reduce the expression of S100P in pancreatic cancer cell line SW1990,and can inhibit the cell growth.

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Available abstract

[Purpose] To investigate the growth inhibition effect of S100P gene silencing by siRNA on human pancreatic cancer cell line SW1990.[Methods] The S100P gene silencing by siRNA was chemically synthesized and transfected into SW1990 cells.The cells were collected at 48h later,and the total RNA and protein was extracted.The mRNA and protein expression of S100P were detected by real-time quantitative PCR and Western blot.The cell growth curve was analyzed by MTT assay.[Results] After transfected with siRNA,S100P mRNA and protein of SW1990 cells were significantly decreased,with inhibition rate of 77% and 74% respectively.The cell growth was significantly inhibited.[Conclusion] S100P gene silencing by siRNA can reduce the expression of S100P in pancreatic cancer cell line SW1990,and can inhibit the cell growth.

Key concepts: Gene silencing, Transfection, Molecular biology, Cell growth, Cell culture, Small interfering RNA, Growth inhibition, Messenger RNA

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The Growth Inhibition Effect of S100P Gene Silencing by SiRNA on Pancreatic Cancer Cell Line SW1990 — Research Paper | ScholarLens