Role of Inflammatory Factor in Brain Damage with Cerebral Ischemia Rat Caused by MMP and TIMP-1

【Objective】 To study the content change of TNF-α,IL-1β,MMP-3,MMP-9,and TIMP-1 in the rats after cerebral ischemia and reperfusion.To clarify the role of TNF-α and IL-1β in brain damage with ischemia rat caused by MMP and TIMP-1.【Methods】 The 56 male SD rats were randomly divided into sham-operation group and ischemia 2 h reperfusion 6 h,12 h,24 h,48 h,72 h,7 d group.The local cerebral ischemia reperfusion model was established by intraluminal thread occlusion of the middle cerebral arteries occlusion(MCAO),the level of TNF-α,IL-1β,MMP-3,MMP-9,and TIMP-1 were determined by enzyme-linked immunosorbent assay(ELISA).【Results】 Compared with sham-operation group,the content of TNF-α and IL-1β in brain tissue and blood were significantly higher in reperfusion 6 h(4.38 ± 0.73 vs 2.63 ± 0.14,5.28 ± 0.71 vs 3.46 ± 0.47;22.34 ± 3.56 vs 12.13 ± 4.26,9.56 ± 0.85 vs 4.23 ± 0.83;P 0.05),and reached the peak at the 12th hour after reperfusion,and then fell down.MMP3 and MMP-9 content increased at the 6th hour after reperfusion.Till the 48th hour it reached the peak,and then fell down.The content of TIMP-1 in brain tissue were significantly lower in reperfusion group than that in the sham-operation group.And the content of TNF-α and IL-1β were positively correlated with MMP-3 and MMP-9(P 0.05),negatively correlated with TIMP-1.【Conclusion】 MMP-3 and MMP-9 play an important role in cerebral ischemia-reperfusion injury.Inflammatory factor can increases the production of MMP-3 and MMP-9 in brain tissue,decrease the production of TIMP-1,and further promote the brain damage cause by ischemia reperfusion.