Effect of Arecoline on Proliferation and Expression of S100A7 mRNA in HaCaT

Objective:To investigate the change of proliferation activity of HaCaT cells under different arecoline concentrations and the effect of elevated arecoline concentrations on the expression of S100A7 mRNA in HaCaT.Methods:HaCaT cells were stimulated with certain concentrations for 24 hours.The changes of proliferation avtivity were tested by MTT.Different expressions of S100A7 mRNA were measured by RT-PCR.Results:Arecoline inhibited cell growth at high concentrations(80-320μg/ml).At concentrations of 80,160 and 320μg/ml,arecoline decreased cell number to 89% ,84% and 61% ,compared to control,corresponding to inhibition of proliferation at 11% 、16% and 39% ,respectively.At low concentrations(0-40μg/ml),arecoline was neither promotive nor inhibitive to cell proliferation.Arecoline at high concentrations induced morphologic changes of HaCaT.Arecoline at concentrations of 40-80μg/ml enhanced S100A7 mRNA expression in HaCaT cells.Conclusion:At low concentrations,arecoline was neither promotive nor inhibitive to cell proliferation of HaCaT.Arecoline at high concentrations significantly inhibited cell proliferation of HaCaT.Arecoline was cytotoxic to HaCaT.Arecoline at certain concentrations enhanced S100A7 mRNA expression in HaCaT cells.S100A7 gene and the protein might play an important role in pathogenesis of OSF.