Comparison and Study on Detection of Single Region Antibody and Total Antibodies Response to Hepatitis C Virus

Objective To investigate the significance of detection of shtgle serum antibody response to hepatitis C virus, by using the internal regent of single region antibody EIA and the external regent anti-HCV 3.0. Methods Seventy-five serums of patients with hepatitis C were tested by EIA with different special antigens (C, NS3, NS4 and NS5) encoded by different regions of HCV genome, and total antibodies were also tested by MEIA of anti-HCV 3.0 from Abbott Co. HCV-RNA. Results In 75 serums, the positive rates of anti-HCV-NS3, anti-HCV-C, anti-HCV-NS4 and anti HCV-NS5 were 93.3% (70/75), 92.0% (69/75), 70.7% (53/75) and 64.0% (48/75), respectively. The positive rates of the total anti-HCV and HCV-RNA were 98.7%(74/75) and 77.4%(58/75). Conclusion The positive rate of different regions of antibodies response to hepatitis C virus is different. Anti-HCV-NS3 and anti-HCV-C are higher. Anti-HCV-NS4 and anti-HCV-NS5 are secondary. Two kinds of regent have a good correlation. Single region anti-HCV EIA may be the additional detection of antibodies of HCV. It is significant that single antiboody being positive. Monitoring the levels of anti-HCV-NS3 and anti-HCV-NS4 can deduce the curative effect of interferon.