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2004•Immunological JournalRequires access

Preparation and characterization of rabbit antiserum against human DOC-2

Shujuan Liu

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Abstract

Objective To prepare antiserum against human DOC-2 using fusion protein GST-nDOC-2. Methods E.coli DH5α transformed with the recombinant plasmid pGEX-4T-nDOC-2 was induced by IPTG to express fusion protein GST- nDOC-2. Antiserum against hDOC-2 was prepared by immunizing rabbit with the fusion protein GST- nDOC-2. The antiserum was identified by ELISA, Western blot, and immunohistochemical staining. Results The antiserum titer was 1∶32 000. Western blotting showed a positive band. The hDOC-2 was detected in normal human ovarian tissue and ovarian serous cystadenoma, but not in ovarian serous cystadenocarcinoma. Conclusion The rabbit antiserum against hDOC-2 is prepared successfully.

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What this paper is about

Objective To prepare antiserum against human DOC-2 using fusion protein GST-nDOC-2. Methods E.coli DH5α transformed with the recombinant plasmid pGEX-4T-nDOC-2 was induced by IPTG to express fusion protein GST- nDOC-2. Antiserum against hDOC-2 was prepared by immunizing rabbit with the fusion protein GST- nDOC-2. The antiserum was identified by ELISA, Western blot, and immunohistochemical staining. Results The antiserum titer was 1∶32 000. Western blotting showed a positive band. The hDOC-2 was detected in normal human ovarian tissue and ovarian serous cystadenoma, but not in ovarian serous cystadenocarcinoma. Conclusion The rabbit antiserum against hDOC-2 is prepared successfully.

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Available abstract

Objective To prepare antiserum against human DOC-2 using fusion protein GST-nDOC-2. Methods E.coli DH5α transformed with the recombinant plasmid pGEX-4T-nDOC-2 was induced by IPTG to express fusion protein GST- nDOC-2. Antiserum against hDOC-2 was prepared by immunizing rabbit with the fusion protein GST- nDOC-2. The antiserum was identified by ELISA, Western blot, and immunohistochemical staining. Results The antiserum titer was 1∶32 000. Western blotting showed a positive band. The hDOC-2 was detected in normal human ovarian tissue and ovarian serous cystadenoma, but not in ovarian serous cystadenocarcinoma. Conclusion The rabbit antiserum against hDOC-2 is prepared successfully.

Key concepts: Antiserum, Molecular biology, Fusion protein, Western blot, Recombinant DNA, Blot, Titer, Biology

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