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2006•Chinese Journal of Appplied Environmental BiologyRequires access

Analysis of Microbial Diversity and Community Composition After Enrichment with Deodorizing Biofilter by DG-DGGE

Tong-sheng Chen

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Abstract

V3 variable fragments of genes coding for 16S rRNA were amplified by Polymerase Chain Reaction. PCR-DG-DGGE combined technique was used to analyze the microbial population composition in different spaces of deodorizing biofilter and difference in community compositions after enrichment in ordor to understand the type and quantity of culture bacteria. Then, some primary DNA fragments were recovered for sequence analysis. The results indicated that there were prominent difference spatially in microbial diversity and dramatic difference between uncultured and enriched bacteria in different media. Sequence alignment results showed that the sulfide-oxidizing bacteria were the predominant population in biofilter. Fig 4, Tab 2, Ref 18

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What this paper is about

V3 variable fragments of genes coding for 16S rRNA were amplified by Polymerase Chain Reaction. PCR-DG-DGGE combined technique was used to analyze the microbial population composition in different spaces of deodorizing biofilter and difference in community compositions after enrichment in ordor to understand the type and quantity of culture bacteria. Then, some primary DNA fragments were recovered for sequence analysis. The results indicated that there were prominent difference spatially in microbial diversity and dramatic difference between uncultured and enriched bacteria in different media. Sequence alignment results showed that the sulfide-oxidizing bacteria were the predominant population in biofilter. Fig 4, Tab 2, Ref 18

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Available abstract

V3 variable fragments of genes coding for 16S rRNA were amplified by Polymerase Chain Reaction. PCR-DG-DGGE combined technique was used to analyze the microbial population composition in different spaces of deodorizing biofilter and difference in community compositions after enrichment in ordor to understand the type and quantity of culture bacteria. Then, some primary DNA fragments were recovered for sequence analysis. The results indicated that there were prominent difference spatially in microbial diversity and dramatic difference between uncultured and enriched bacteria in different media. Sequence alignment results showed that the sulfide-oxidizing bacteria were the predominant population in biofilter. Fig 4, Tab 2, Ref 18

Key concepts: Biofilter, Bacteria, 16S ribosomal RNA, Population, Microbial population biology, Biology, Food science, Microbiology

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