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2003•Zhongguo nongye KexueRequires access

Analysis of Expressed Sequence Tags (ESTs) in White Fat Tissue of Pig

Liu Zhao

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Abstract

2 104 expressed sequence tags(ESTs) from a cDNA library of porcine adipocyte were studied. The results showed that the nucleotide sequences of 563 ESTs have already been presented in GenBank database, 682 ESTs could be found homologous with those of human and other species, while 376 ESTs were not identified. 298 ESTs were e-liminated for the poor quality of sequence, and 185 ESTs have their homologues in the EST database. Of the 376 ESTs, 159 have ORF structure, and 43 contain Poly (A) or/and CpG elements, and 174 had no above structures or elements. All these results preliminarily showed the expression style of functional genes in porcine adipocyte.

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What this paper is about

2 104 expressed sequence tags(ESTs) from a cDNA library of porcine adipocyte were studied. The results showed that the nucleotide sequences of 563 ESTs have already been presented in GenBank database, 682 ESTs could be found homologous with those of human and other species, while 376 ESTs were not identified. 298 ESTs were e-liminated for the poor quality of sequence, and 185 ESTs have their homologues in the EST database. Of the 376 ESTs, 159 have ORF structure, and 43 contain Poly (A) or/and CpG elements, and 174 had no above structures or elements. All these results preliminarily showed the expression style of functional genes in porcine adipocyte.

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Available abstract

2 104 expressed sequence tags(ESTs) from a cDNA library of porcine adipocyte were studied. The results showed that the nucleotide sequences of 563 ESTs have already been presented in GenBank database, 682 ESTs could be found homologous with those of human and other species, while 376 ESTs were not identified. 298 ESTs were e-liminated for the poor quality of sequence, and 185 ESTs have their homologues in the EST database. Of the 376 ESTs, 159 have ORF structure, and 43 contain Poly (A) or/and CpG elements, and 174 had no above structures or elements. All these results preliminarily showed the expression style of functional genes in porcine adipocyte.

Key concepts: Expressed sequence tag, GenBank, Biology, Complementary DNA, cDNA library, Sequence (biology), Gene, Genetics

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