Inhibition of siRNA Targeting MMP-9 on Ivasion and Migration of Human SGC7901 Gastric Adenocarcinoma Cells

Objective:To investigate the effect of MMP-9 siRNA on MMP-9 expression of human SGC7901 gastric adenocarcinoma cells and the inhibition effect on SGC7901 cell invasion and migration thereof.Methods:To design and synthesize siRNA targeting MMP-9,then transfect it into SGC7901 gastric adenocarcinoma cells with oligofectamine.The realtime PCR and Western blotting were used to observe the expression of MMP-9 mRNA and the protein.The Matrgel 3D growth experiment and transwell experiment were used to detect the invasion ability of cells.The Matrgel 2D growth experiment and scratch experiment were used to detect the migration ability of SGC7901 cells.Results:Real-time PCR and Western blotting assay indicated that the expressions of MMP-9 mRNA and the protein in SGC7901 cells interfered by MMP-9 siRNA were significantly lower than those of other two groups.The invasion and migration ability of SGC7901 cells were also degraded after siRNA interference of MMP-9 gene.Conclusion:The targeting RNA interference not only degraded the expression of MMP-9 mRNA and protein,but also inhibited the invasion and migration ability of SGC7901 cells.