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Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effects on rat airway smooth muscle cells

Ying Liu

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Abstract

AIM: To observe the antiproliferative effects of antisense recombinant adenoviral vector for c myc on rat airway smooth muscle cells. METHODS: Antisense and sense bacterial plasmids for c myc were constructed. Bacterial plasmids and E1 deleted adrenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTT and the expression of c Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense recombinant adenoviral vector for c myc could inhibit rat airway smooth muscle cells proliferation. The expression of c Myc decreased after antisense recombinant adenoviral vector for c myc was transfected into cells. CONCLUSION: Recombinant adenoviral vector antisense for c myc can inhibit rat airway smooth muscle cells proliferation.

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What this paper is about

AIM: To observe the antiproliferative effects of antisense recombinant adenoviral vector for c myc on rat airway smooth muscle cells. METHODS: Antisense and sense bacterial plasmids for c myc were constructed. Bacterial plasmids and E1 deleted adrenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTT and the expression of c Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense recombinant adenoviral vector for c myc could inhibit rat airway smooth muscle cells proliferation. The expression of c Myc decreased after antisense recombinant adenoviral vector for c myc was transfected into cells. CONCLUSION: Recombinant adenoviral vector antisense for c myc can inhibit rat airway smooth muscle cells proliferation.

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Available abstract

AIM: To observe the antiproliferative effects of antisense recombinant adenoviral vector for c myc on rat airway smooth muscle cells. METHODS: Antisense and sense bacterial plasmids for c myc were constructed. Bacterial plasmids and E1 deleted adrenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTT and the expression of c Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense recombinant adenoviral vector for c myc could inhibit rat airway smooth muscle cells proliferation. The expression of c Myc decreased after antisense recombinant adenoviral vector for c myc was transfected into cells. CONCLUSION: Recombinant adenoviral vector antisense for c myc can inhibit rat airway smooth muscle cells proliferation.

Key concepts: Recombinant DNA, Transfection, Molecular biology, Viral vector, Sense (electronics), Vector (molecular biology), Biology, Plasmid

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Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effects on rat airway smooth muscle cells — Research Paper | ScholarLens