Effects of mature dendritic cells derived from mouse bone marrow on T cells proliferation in vitro

Objective To study the effects of mature dendritic cells (DCs) derived from mouse bone marrow on T cell proliferation in vitro , and provide techinological methods for tumor therapy.Methods With IL 4 and GM CSF, the cells from mouse bone marrow were cultured for 5 7 days. The morphological changes were observed under light microscope on 3rd and 5th day after culture; immature DCs on 5th or 6th day after culture were loaded with tumor antigen for 1 2 days; CD83 and CD86 were tested by flowcytometric analysis; T cell stimulatory activity in DCs derived from mouse bone marrow and loaded with tumor antigen was analysed by γ scintillomete. Results Morphological changes were found and dendritic protrusion appeared on 3rd day after culture; dendritic protrusion prolonged on 5th day after culture; flow cytometric analysis showed that expressions of CD83 and CD86 of IL 4+GM CSF+TNFα group (61 68% and 71 25%) and IL 4+GM CSF+tumor antigen group (63 1% and 76 88%) had significant differences compared with control group (2 41% and 3 88%) and IL 4+GM CSF group (21 86% and 28 69%) ( P 0 001), and IL 4+GM CSF+tumor antigen group had also significant difference compared with IL 4+GM CSF+TNFα group( P 0 01); proliferation of T cells in IL 4+GM CSF+tumor antigen group strongly enhanced compared with other groups ( P 0 001, P 0 05). Conclusion Sufficient DCs generated from bone marrow cells are induced in vitro and strongly stimulate proliferation of T cells after tumor antigen are loaded.