ScholarLens Back to search
2015•Unpublished venueRequires access

Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis

Xiao Hu

Open publisher page 0 citations

Abstract

Zinc-finger is a protein that binds DNA / RNA and regulates gene expression. In previous work,we identified a Zinc-finger protein( termed as Hb Zinc-finger) that interacted with Hb JAZ1 in Y2 H against rubber latex c DNA yeast library. The full-length c DNA of Hb Zinc-finger gene is 2 262 bp in length and encodes 753 aa.C-terminal of Hb Zinc-finger contains a PB1 domain. A Zn F_ZZ domain exists between 336 aa and 381 aa. Subcellular fluorescent localization result showed that the Hb Zinc-finger was located in nucleus. Additionally,Hb Zinc-finger showed strong self transcriptional activation in yeast. Furthermore,the expression of Hb Zinc-finger was affected by continuously wounding in rubber latex,suggesting that Hb Zinc-finger is possibly regulated by the JA signaling pathway.

About this research paper

What this paper is about

Zinc-finger is a protein that binds DNA / RNA and regulates gene expression. In previous work,we identified a Zinc-finger protein( termed as Hb Zinc-finger) that interacted with Hb JAZ1 in Y2 H against rubber latex c DNA yeast library. The full-length c DNA of Hb Zinc-finger gene is 2 262 bp in length and encodes 753 aa.C-terminal of Hb Zinc-finger contains a PB1 domain. A Zn F_ZZ domain exists between 336 aa and 381 aa. Subcellular fluorescent localization result showed that the Hb Zinc-finger was located in nucleus. Additionally,Hb Zinc-finger showed strong self transcriptional activation in yeast. Furthermore,the expression of Hb Zinc-finger was affected by continuously wounding in rubber latex,suggesting that Hb Zinc-finger is possibly regulated by the JA signaling pathway.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Zinc-finger is a protein that binds DNA / RNA and regulates gene expression. In previous work,we identified a Zinc-finger protein( termed as Hb Zinc-finger) that interacted with Hb JAZ1 in Y2 H against rubber latex c DNA yeast library. The full-length c DNA of Hb Zinc-finger gene is 2 262 bp in length and encodes 753 aa.C-terminal of Hb Zinc-finger contains a PB1 domain. A Zn F_ZZ domain exists between 336 aa and 381 aa. Subcellular fluorescent localization result showed that the Hb Zinc-finger was located in nucleus. Additionally,Hb Zinc-finger showed strong self transcriptional activation in yeast. Furthermore,the expression of Hb Zinc-finger was affected by continuously wounding in rubber latex,suggesting that Hb Zinc-finger is possibly regulated by the JA signaling pathway.

Key concepts: Zinc finger, LIM domain, Zinc finger nuclease, RING finger domain, Hevea brasiliensis, Zinc, Gene, DNA

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Characterization of a Zinc-finger Gene of Hevea brasiliensis — Research Paper | ScholarLens