Study on 3D-HPLC Characteristics Fingerprints of Coptidis Rhizoma

OBJECTIVE:To analyze the alkaloids component of various Coptidis Rhizoma from different producing areas,and to establish 3D-HPLC characteristics fingerprints.METHODS:HPLC-DAD was adopted.The determination was performed on Xtimate TM C 18(250 mm×4.6 mm,5 μm)column with mobile phase consisted of 30 mmol/L ammonium hydrogen carbonate solution(including 0.1% triethylamine,0.7% ammonia water)-acetonitrile(gradient elution) at the flow rate of 1.0 ml/min.The detection wavelength was set at 200-400 nm and column temperature was 30 ℃.The similarity evaluation and cluster analysis were carried out to obtained fingerprints of 31 batches of Coptis chinensis,6 batches of C.deltoidea,4 batches of C.teeta at 270 nm by similarity evaluation software and SPSS18.0 software.RESULTS:3D-HPLC-DAD fingerprints of Coptidis Rhizoma were set up to obtain 11 characteristic fingerprint peaks;the common peak area took up over 90% of total peak area.7 kinds of alkaloids were demarcated by comparing with substance control,such as magnoflorine,jateorrhizine hydrochloride,palmatine hydrochloride,epiberberine hydrochloride,coptisine hydrochloride,palmatine hydrochloride and berberine hydrochloride.Content difference of epiberberine hydrochloride was symbolic feature of 3 kinds of Coptidis Rhizoma.CONCLUSION:The method is accurate,stable and reproducible.The established characteristic fingerprints can be taken as one of main criteria of quality evaluation of Coptidis Rhizoma.