Curcumin-induced the expression of inhibitor κBα protein in human prostate cancer cells

Objective To investigate the eurcumin-induced the expression of IκBα in androgen- dependent(LNCaP)and androgen-independent(PC3)prostate cancer cells,and to study the mechanisms of curcumin on the proliferative inhibition of prostate cancer cells.Methods After LNCaP and PC3 cells were affected by 10,25,50,75,100 μmol/L curcumin respectively,the cell activity was assayed with methyl thiazolyl tetrazolium(MTT)method at 5,12 and 24 hours;Flow cytometry was adopted to observe the cell cycle of LNCaP and PC3 cells at 24 hours.After 5 hours,the expression of IκBα in LNCaP and PC3 cells was observed with Western blotting.Results Curcumin obviously suppressed the proliferation of LNCaP and PC3 cells in does-dependent and time-dependent manners.Curcumin could arrest the cell cycle of LNCaP and PC3 cells at G_2 M phase and then induce cell apoptosis.The expression of IκBαin LNCaP cells had no significant difference after using curcumin(F=0.129,P0.05).However,the expression of IκBα in PC3 cells increased gradually with the inducement of concentration-increased curcumin(F= 31.618,P0.05).Conclusions IκBα may play a role in the eureumin inducing apoptosis of PC3 cell, while the curcumin inducing apoptosis of LNCaP cells is by antioxidation and inhibiting metabolites formation in LNCaP cells.