A Study of Transfecting Recombinant pEGFP-heNOS Plasmid into Human EPC and Optimizing Its Transfection Conditions

Objective To transfect recombinant plasmid pEGFP-heNOS into human endothelial progenitor cell(EPC) by liposome and to optimize the conditions of transfection. MethodsRecombinant plasmid pEGFP-heNOS was transfected into endothelial progenitor cell by liposome and changed the quantity of plasmid or liposome. The transfection efficiency was observed by fluorescence microscopy and the heNOS expression in endothelial progenitor cell was last detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot. ResultsRecombinant plasmid pEGFP-heNOS was transfected into endothelial progenitor cell successfully, When the proportion of plasmid:liposome was 1:1,the transfection efficiency was the highest. ConclusionRecombinant plasmid pEGFP-heNOS is effectivly expressed after being transfected into endothelial progenitor cell in vitro and it enhanced the transfection efficiency by optimizing the transfection conditions,which provides experimental support for further gene therapy.