2010•Zhongguo sheng-hua yaowu zazhiRequires access
Study on construction of citrostatin and its bioactivity
Ma Li, Cai Zai-long, Qingrong Wang, Lei Cheng-xiang
Open publisher page 1 citations
Abstract
Purpose To construct a recombined antitumor peptide and to analyze its bioactivity.Methods Constructing a recombined gene and inserting the pGEX-4T-3 vector.The recombined protein was expressed in E.coli BL21 and purified with Amylose Resin.Then,citrostatin was subjected to the following tests separately:inhibition of endothelial cell proliferation,MTT test of cytotoxicity and inhibition of endothelial cell tube formation on ECMatrix.Results Citrostatin significantly inhibited the proliferation of human endothelial cell ECV304(IC50=2.28 μmol/L).It also significantly inhibited the proliferation of human tumor cell 1990 and NCI-H640(IC50=9.24,2.74 μmol/L),and the inhibitory effect became more marked with the increase of citrostatin concentration.The inhibitory effects of citrostatin on endothelial cell tube formation was also confirmed.Conclusion An antitumor peptide,citrostatin,has been successfully constructed and purified,which showed anti-angiogenesis effect and direct cytotoxic effect on tumor cells.