2009•Hubei nongye kexueRequires access
Optimization of RAPD-PCR Reaction Conditions for Sweet Potato
Xinsun Yang
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Abstract
In this paper,CTAB method was used to extract genomic DNA from sweet potato Xushu No.22.High purity DNA was selected as DNA template.Use random primers to run RAPD-PCR reaction.Through setting a gradient concentrations of the Taq DNA polymerase,MgCl2,dNTPs,DNA template and primers,the suitable conditions for RAPD-PCR reaction of sweet potato was obtained: 25.0 μL reaction system which contains 2.5 μL PCR 10×Buffer,0.15U Taq DNA polymerase;3.0 nmol·μL-1 MgCl2;0.6 nmol·μL-1 dNTPs;40 ng DNA template and 40 ng primer.