The Research of Anti-Proliferation and Induction of Apoptosis of Resveratrol on Human oral Squamous Cell Carcinoma KB Cells in Vitro

Objective:To study the resveratrol on human oral squamous cell carcinoma cells proliferation and apoptosis.Methods:MTT colorimetric assay of KB cell in vitro growth inhibition;Cell cycle phase distribution and hypodiploid DNA was determined by flow cytometry;Hoechst33258 fluorescent dye staining,indicating apoptotic cell morphology;Western-blot method of detection of survivin,Caspase-3,Smac protein expression.Results:Res able to dose-and time-dependent manner inhibited KB cell growth;A dose dependent manner makes KB cell cycle arrest in S phase and induce apoptosis;Hoechst33258 staining,showing characteristics of apoptosis cells were significantly;Western-blot method measured Res down Survivin protein expression in KB cells,and increase the KB cell cytoplasm Smac protein content(P0.01),Caspase-3 protein was activated.Conclusion:Res inhibits proliferation of KB cell,the cell cycle arrest was S can induce apoptosis,Survivin,Caspase-3 and Smac gene involved in apoptosis of KB cell.