2006•PubMedRequires access

[Adriamycin enhances anti-human DR5 monoclonal antibody (mDRA-6) induced HL-60 cells apoptosis].

Shulian Li, Yuanfang Ma, Guangchao Liu, Jun Zhang, Hui-Ling Bai, Yingjie Liu, Lu Feng

Abstract

OBJECTIVE: To investigate synergistic killing effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody (mDRA-6) and adriamycin(Adr) on HL-60 cells. METHODS: mDRA-6 was prepared by immunizing BALB/c mice with DR5 protein. DR5 expression on Adr-treated HL-60 cells was detected by flow cytometry. Morphologic changes of HL-60 cells were observed under fluorescence microscope. Cytotoxic and apoptotic effects of mDRA-6 and Adr on HL-60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis. RESULTS: Adr induce DR5 expression on HL-60 cells. Cell budding, chromatin condensation and apoptotic body formation were observed in HL-60 cells treated by mDRA-6 and Adr. Death and apoptosis of these cells and DNA ladder were exhibited on agarose gel electrophoresis. CONCLUSION: mDRA-6 and Adr have synergistic killing effect on HL-60 cells.

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Key concepts: Chemistry, Agarose gel electrophoresis, Apoptosis, Molecular biology, Flow cytometry, MTT assay, Cytotoxic T cell, Fragmentation (computing)