2008•Huadong Shifan Daxue xuebao. Ziran kexue banRequires access
Differentiation of different explants and enlargement of tissue culture bulb of narcissus at Chongming (Narcissus tazetta var. chinensis) (Chinese)
Xiong Lijun, Xiaofang Li, Yang Wang, Sun Yue, Ling Xu
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Abstract
This paper studied the de-differentiation and differentiation of various explants such as bulb,leaf and infancy inflorescence of narcissus(Narcissus tazetta var.chinensis).The results showed that infrastructure and infancy inflorescence of narcissus were the best explants.When the infrastructure of adventitious buds was used as explants,the quantity of buds increased three times in forty days.Additionally,it was found that KH2PO4 of certain concentration and dark conditions were favorable to enlargement adventitious buds.The anatomic structure of shoot apical meristem of bulb from field was different from that of tissue culture bulb,which might be the reason why tissue culture bulbs were very hard to enlarge.