Study on Separation and Purification for CoQ_(10) by Column Chromatography

Coenzyme Q10 which was extracted by acetone grinding from peanut bud were separated and purified by thin layer chromatography and silica gel column chromatography.The results showed that sample solution was applied to silica gel G plates,while mixture of petroleum ether/aether(6/1,v/v) was used as mobile phase.After detected in the iodine-cylinder,there appeared five yellow spots.The optimum condition for silica gel column were confirmed:0.5mL of extraction solution from peanut bud was applied to silica gel column 10mm×600mm(60~100 mesh) while mixture of petroleum ether/aether(6/1,v/v) was used as mobile phase.The flow rate was 2.0 mL/min,collect No.36~50 mins eluent.The most content of CoQ10 was 92.96%.