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2006•Military Medical Journal of South ChinaRequires access

Study of Effect of Cydosporin A on Gene Differential Expression in Livers by cDNA Microarray in Rats

Yu Ai

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Abstract

Objective To study the effects of cyclosporin A (CsA) on genes expression in rat liver. Methods The rats were sacrificed 24 hours after being orally treated once with cycolsporin A and the livers were taken out to extract total RNA. The cDNA derived from negative control group and CsA group livers were labded with Cy3 - dCTP and Cy5 - dCTP respectively. These probes were hybridized with cDNA microarray. The acquired image was scanned and analyzed by GenePix Pro 3.0 software. The intensity ratio of Cy5 to Cy3 about each spot was calculated. Results Among 4 096 genes, 50 genes exhibited differential expression in CsA group ,of which, 17 genes were up - regulated and 33 genes down - regulated. Conclusions The immuno - inhibitive effect, anti - cancer effects and interactions between CsA and other drugs may be related with the changes in the expressions of some associated genes.

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Objective To study the effects of cyclosporin A (CsA) on genes expression in rat liver. Methods The rats were sacrificed 24 hours after being orally treated once with cycolsporin A and the livers were taken out to extract total RNA. The cDNA derived from negative control group and CsA group livers were labded with Cy3 - dCTP and Cy5 - dCTP respectively. These probes were hybridized with cDNA microarray. The acquired image was scanned and analyzed by GenePix Pro 3.0 software. The intensity ratio of Cy5 to Cy3 about each spot was calculated. Results Among 4 096 genes, 50 genes exhibited differential expression in CsA group ,of which, 17 genes were up - regulated and 33 genes down - regulated. Conclusions The immuno - inhibitive effect, anti - cancer effects and interactions between CsA and other drugs may be related with the changes in the expressions of some associated genes.

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Available abstract

Objective To study the effects of cyclosporin A (CsA) on genes expression in rat liver. Methods The rats were sacrificed 24 hours after being orally treated once with cycolsporin A and the livers were taken out to extract total RNA. The cDNA derived from negative control group and CsA group livers were labded with Cy3 - dCTP and Cy5 - dCTP respectively. These probes were hybridized with cDNA microarray. The acquired image was scanned and analyzed by GenePix Pro 3.0 software. The intensity ratio of Cy5 to Cy3 about each spot was calculated. Results Among 4 096 genes, 50 genes exhibited differential expression in CsA group ,of which, 17 genes were up - regulated and 33 genes down - regulated. Conclusions The immuno - inhibitive effect, anti - cancer effects and interactions between CsA and other drugs may be related with the changes in the expressions of some associated genes.

Key concepts: Complementary DNA, Microarray, Gene, Gene expression, Molecular biology, Biology, Microarray analysis techniques, RNA

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