miR-34aregulates high glucose-induced apoptosis of H9c2cells

Objective To study the effect of miR-34aon regulation of high glucose-induced apoptosis of H9c2cells.Methods Cultured H9c2cells were randomly divided into normal glucose(5.5mmol/L)group,high glucose(33mmol/L)group and miR-34ainhibitor(50nmol/L)+glucose(33mmol/L)group.Expressions of miR-34aand Bcl-2mRNA were detected by RT-PCR.Western blot was used to monitor the changes in apopotosis-associated protein Bcl-2,respectively.Apoptosis of-H9c2cells was detected by flow cytometry.Results The apoptpsis level of H9c2cells and the expression level of miR-34ain H9c2cells were significantly higher whereas the expression level of Bcl-2was significantly lower in high glucose group than in normal glucose group(P0.05).The apoptpsis level of H9c2cells was significantly lower whereas the expression level of Bcl-2was significantly higher in miR-34ainhibitor+glucose group than in high glucose group(P0.05).Conclusion miR-34aregulates high glucose-induced apoptpsis of H9c2cells by inhibiting the expression of Bcl-2.