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2015•Fujian nongye xuebaoRequires access

Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)

Fang Zhi-zhe

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Abstract

A 1309 bp full length cDNA sequence of PsACO1 gene were isolated from Furong plum(Prunus salicina Lindl.)through RT-PCR and RACE technology.The isolated gene contains a 957 bp open reading frame and encodes a protein of 319 amino acids with isoelectric point of 5.28 and molecular weight of 36.24 kD.PsACO1protein contains characteristic domains of ACO proteins.The phylogenetic analysis results showed that PsACO1 has close corelation with ACO protein fromPrunus persica,Prunus mume,Eriobotryajaponica,Malus domestica and Prunus domestica.

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What this paper is about

A 1309 bp full length cDNA sequence of PsACO1 gene were isolated from Furong plum(Prunus salicina Lindl.)through RT-PCR and RACE technology.The isolated gene contains a 957 bp open reading frame and encodes a protein of 319 amino acids with isoelectric point of 5.28 and molecular weight of 36.24 kD.PsACO1protein contains characteristic domains of ACO proteins.The phylogenetic analysis results showed that PsACO1 has close corelation with ACO protein fromPrunus persica,Prunus mume,Eriobotryajaponica,Malus domestica and Prunus domestica.

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Available abstract

A 1309 bp full length cDNA sequence of PsACO1 gene were isolated from Furong plum(Prunus salicina Lindl.)through RT-PCR and RACE technology.The isolated gene contains a 957 bp open reading frame and encodes a protein of 319 amino acids with isoelectric point of 5.28 and molecular weight of 36.24 kD.PsACO1protein contains characteristic domains of ACO proteins.The phylogenetic analysis results showed that PsACO1 has close corelation with ACO protein fromPrunus persica,Prunus mume,Eriobotryajaponica,Malus domestica and Prunus domestica.

Key concepts: Prunus salicina, Prunus, Open reading frame, Gene, Complementary DNA, Biology, Cloning (programming), Botany

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Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.) — Research Paper | ScholarLens