Large Culures for Haematococcus pluvialis Outside and Accumulation of Astaxanthin

The green microalga Haematococcus pluvialis was cultured outside in large volume,the effects of different trophic and stress conditions on cell growth and astaxanthin accumulation were studied.The effects of different strains on accumulating astaxanthin was also investigated.The results showed that the growth rate of the strain HPM outside was 0.22,and the astaxanthin production was 1.35 mg/L,which were higher than the other two strains.The stress period was shortened under the mixotrophic system with addition of NaAc,and the astaxanthin content moreased 126.3% than that under the autotrophic system.The stress of high salinity was better to accumulate astaxanthin than high light intensity.Under the stress of high salinity,the astaxanthin production was 1.33 mg/L compared to the data under the stress of high light(0.76 mg/L).