Isolation and cultivation of mouse peritoneal macrophages and detection of phagocytic function

Objective:To establish a method for isolating and culturing mouse peritoneal macrophages in vitro,and detect the phagocytic function in vivo.Methods:Physiological saline solution containing 5% starch was used to produce peritoneal macrophages in the mouse abdominal cavity,and then the cells were separated and cultured to observe cell morphology in vitro.Taking 5% chicken red cell suspension as phagocytic object,phagocytosis percentage and phagocytosis index were determined in vivo.Results:The macrophage livability excelled 95%,showing better morphology and forming better cell monolayer,and the average phagocytosis percentage and phagocytosis index were 26% and 0.28.Conclusion:The method can be used for studies on the cultivation of mouse peritoneal macrophages.