Modulating functions of icariin emodin and baicalin on bone resorption caused by osteoclasts in vitro

Objective To investigate the modulating functions of icariin emodin and baicalin on bone resorption caused by osteoclasts.Methods In this protocol,osteoclasts were separated from long limb bones of new born rabbits,cultured with deactivated human tooth slices and glass slices,10 μg/ml IL-1β and 10 μg/ml emodin,0.01 μg/ml baicalin,0.01 μg/ml icariin or their combination.The quantity and area of the bone resorption on the slices were observed and measured.Results Several resorption pits were founded on the slices after cultured for 24 hours with the separated cells from long limb bones of newborn rabbits.After 10 hours,the numbers and area of the resorption pits on the treated tooth slices were all significantly lower than those in the control groups(P0.05),and the function of the combination group was more significant(P0.01).Conclusion The results indicated that the combination of icariin emodin and baicalin could inhibit bone resorption caused by osteoclasts.