Effect and mechanism of Tongxinluo on ischemia/reperfusion injury through brain slices

Objective To explore the study and mechanism of Tongxinluo on ischemia/reperfusion injury through organotypic brain slices.Methods Brain slices(300 μm) from postnatal 3rd day SD rats were prepared and randomly divided into 2 groups: ischemia/reperfusion group and normal group.After ischemia/reperfusion injury,they were divided into 4 groups(high-dose Tongxinluo group,low-dose Tongxinluo group,control group and normal saline group).Slices were observed under invert microscope or detected by immunofluorescence staining.Results Neonatal cells were labeled with nestin in the subventrical zone and hippocampal dentate gyrus when brain slices were treated with different dosage of Tongxinluo,while ischemia/reperfusion group had less cells(P0.05).After cultured for 1 day,3 days and 7 days,the number of neonatal cells decreased in the order of high-dose Tongxinluo group,low-dose Tongxinluo group,control group and normal saline group(P0.05).The density of neonatal cells in the subventrical zone and hippocampal dentate gyrus decreased due to cell migration with time.Each group had many neonatal cells labeled with nestin at 1st day,but only two Tongxinluo groups had some neonatal cells labeled with nestin at 7th day.Conclusion Tongxinluo can be used to treat ischemia/reperfusion injury by inducing the proliferation,migration and differentiation of neural stem cells in a close-dependent wanner.