Determination of 15 β-agonists Residues in Pork by Ultra-performance Liquid Chromatography-mass Spectrometry

[Objective]To develop a method for determination of 15β-agonists residues in pork by ultra-performance liquid chromatography-mass spectrometry.[Method]The samples were enzyme hydrolyzed by β-Glucuronidase/aryl sulfatase,etracted with 0.2 mol/L Acetic acid-ammonium acetate buffering solution(p H=5.0),and cleaned up on a SPE cartridge,separated on a C18 column using 0.01mol/L ammonium acetate(contain 0.1% Formic Acid)- Methanol as mobile phase,and detected under multiple reaction monitoring(MRM)mode.[Results]The resultes displayed a good linearity for15β-agonists over the range of 5~100 ng/m L.The mean recoveries were from 74.2% to 115.6% at three different concentrations.[Conclusion]The method was precise,sensitive,and highly efficient in extraction and purification,and it is suitable for fast analysis of β-agonists residues in a large number of pork samples.