Experimental Study on Berberine on Apoptosis and Regulation gene in Human Gastric Cancer Cell

Objective To study the Effect of berberine on apoptosis and P 53 ,Bcl-2 gene transcription in human gastric cancer cell SGC-7901. The apoptosis of gastric cancer cells were detected by scanning electron microscope and flow cytometry. The mRNA expression of P 53 and Bcl-2 were assayed with RT-PCR. After 72 hours exposure to berberine of 8ug/l,apoptotic bodies were detected. There were apoptotic peaks in DNA histogram in berberine of 4ug/l and 8ug/l. The Bcl-2 mRNA transcription decreased in 4ug/l and there was no Bcl-2 in 4ug/l and 8ug/l. The wild type P 53 mRNA transcription elevated in both doses. [Conclusion]Berberine can induce apoptosis in human gastric cancer cell. The 1mechanism is to reduce the transcription of Bcl-2 and increase the P 53 . And it has the potential value in treating gastric cancer.