Effect of Ischemic Postconditioning on Oxidative Stress Induced by Renal Ischemic Reperfusion Injury in Rats

Objective To investigated the protective effect of ischemic postconditioning(IPostC) against oxidative stress induced by renal ischemic reperfusion injury in rats.Methods After right nephrectomy,rats were subjected to 45 min of left renal ischemia followed by 24 h reperfusion.IPostC was conducted by six cycles of 10 sec reperfusion/10 sec renal pedicle occlusion at the end of ischemia,followed by 6 h reperfusion.function was evaluated by the levels of serum creatine and urea nitrogen;the renal pathologic change was observed by HE staining;the concentration of MDA and the activity of SOD were measured by spectrophotometric method;The mRNA and protein expression of SOD were evaluated by Real Time RT-PCR and Western blotting,respectively.Results Our data showed that ischemic postconditioning decreased the levels of serum creatine and urea nitrogen and improved the renal pathologic change.Ischemic postconditioning attenuated significantly the levels of MDA caused by ischemic reperfusion(P0.05),and increased the activity,mRNA and protein expression of SOD(P0.05).Conclusion Ischemic postconditioning can improve renal function.The mechanism of ischemic postconditioning against renal IRI may be related to antioxidative effect on the lipid peroxidation,increasing the activity and expression of antioxidative enzyme,which improve antioxidative ability of kidney.