2007•Shiyong yixue zazhiRequires access
Expression of local angiotensinogen mRNA and activation of local nuclear factor-κB in vasculopathy of portal hypertension
Zhe Yang
Open publisher page 0 citations
Abstract
Objective To investigate the expression of local angiotensinogen mRNA and activation of local nuclear factor-κB in vasculopathy of portal hypertension(PHT) and to discuss their role in the pathogenesis of portal hypertensive vasculopathy.Methods The expression of local angiotensinogen mRNA in splenic artery and vein of PHT patients and normal vascular were detected by RT-PCR analysis.Chemiluminescent electrophoretic mobility shift assay was used to detect the activation of NF-κB in splenic artery and vein.Result Levels of local angiotensinogen mRNA in splenic artery and vein in PHT group were significantly higher than those in control group(0.48 ± 0.21 vs 0.23 ± 0.12 and 0.43 ± 0.16 vs 0.18 ± 0.10,both P 0.05).There was no significant difference of the expression of local angiotensinogen mRNA between the splenic artery and vein in PHT group.The activation of NF-κB in splenic artry and vein in PHT patients was significantly higher than that in control group(P 0.05).The expression of local angiotensinogen mRNA in splenic artery and vein in PHT patients had a significantly positive correlation with the activity of NF-κB(r=0.692,P 0.05;r=0.703,P 0.05,respectively).Conclusion Local angiotensinogen and activation of NF-κB may be one of the pathogenic factors of portal hypertensive vasculopathy.