The change of Nestin and bFGFmRNA after middle cerebral artery occlusion in rats

Objective To investigate the activation of BrdU+Nestin、bFGF after middle cerebral artery oc- clusion in rats.Methods Ischemia was induced by temporary middle cerebral artery occlusion(MCAO),which was divided into the sham operation group,MCAO group.In subventricle zone(SVZ) and subdentate gyrus zone(SGZ) of rats at 3,5,7,14,21 and 30 days after MCAO,the numbers of BrdU+Nestin were detected by immunohis- tochemistry and the bFGFmRNA were detected by RT-PCR.Results Almost there were not BrdU+NestFmRNA in the sham operation group.The BrdU+Nestin and bFGFmRNA was significantly difference compared in the 2 group.BrdU+Nestin and bFGFmRNA had significantly distincted in ischemia zone of MCAO at the each time point (P0.05).BrdU+Nestin were significantly increased on the 14th day.The bFGFmRNA began heighten on 3th day,was the highest on 7th day,and turned to basic line on 30th day.Conclusions This study demonstrates that focal cerebral ischemia in the rat results in a rapid response,a process often referred to as reactive Nestin in the side of ischemia and the ability of the differentiation and proliferation of neural stem cells breed in the MCAO. The differentiation and proliferation of neural stem cells is related with bFGFmRNA expression.