Study on Subunit B/Whole Cell Oral O139 Cholera Vaccine

[Abstract] Objective To prepare oral subunit B/whole cell vaccine for preventing cholera caused by strain 0139.Methods The subunit B/ whole cell vaccine was prepared with the subunit B of recombinant cholera toxin and the 0139 strains of vibrio cholerae inactivated with formalin,and the safety and immunogenic-ity of it were studied. Results No bodyweight loss, morbidity or death were observed in the mice immunized with the oral vaccine at large dosages for 3 days. All the mice were survival and healthy. The specific antibody of high liters were detectecd out in the peripheral blood and intestinal tract of mice immunized with the vaccine by oral route or I. P. injection. The vaccine protected the mice against the challenge with live 0139 strain. Conclusion The subunit B/whole cell vaccine showed good safety and immunogenicity in mice.