The Effect of Mycobacterium Tuberculosis Hsp16.3 on Apoptosis of Infected Macrophages

Objective:To investigate the effect of Mycobacterium tuberculosis Hsp16.3on apoptosis of infected macrophages.Methods:RAW264.7were infected with H37Rv,△H37Rv,BCG and△BCG and the apoptotic rate of the infected macrophages were detected by flow cytometry at 1h,3h,6hand 12hafter infection,respectively.Results:The apoptotic rate of the infected macrophages were increased significantly,especially the apoptotic rate of macrophages in BCG group were higher than H37Rv group(P0.05)at 1h,3hand 12hafter MTB infection;the apoptotic rate of macrophages in △BCG group were higher than△H37Rv group(P0.05)at 1hand 3hafter MTB infection;at 3h,6hand 12hafter MTB infection,the apoptotic rate of macrophages in△H37Rv group and△BCG group were higher than H37Rv group and BCG group,ordinally(P0.05).Conclusion:Mycobacterium tuberculosis Hsp16.3can restrain the apoptosis of the macrophages infected.