Determination of 9 Organochlorine Pesticides in Licorice Root Medicinal Material by Gas Chromatography

OBJECTIVE:To establish gas chromatography(GC)for simultaneous determination of the contents of 9 organochlorine pesticides in licorice root medicinal material.METHODS:The determination of contents was performed by GC,briefly,capillary column DB-1701(30 m×0.25 mm,0.25 μm)was used,with injection port temperature kept at 220 ℃;column temperature kept at 140 ℃ for 1 min then raised to 210 ℃ at an increasing rate of 10 ℃/min,with the temperature retained for 1 min then raised to 260 ℃ at an increasing rate of 20 ℃/min,with this temperature retained for 20 min;the detector temperature was kept at 300 ℃;the carrier gas was high purity nitrogen(99.999%)at a flow rate of 1.0 ml/min in constant current mode without split flow with injection volume of 1 μl.RESULTS:The linear ranges of the 9 organochlorine pesticides were 1-250 ng/ml.The average recovery rate was 102.40% with RSD of 2.12%(n=9).CONCLUSION:The GC method is simple,rapid and accurate,and it is suitable for the content determination of 9 organochlorine pesticides in licorice root medicinal material.