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2002•Letters in BiotechnologyRequires access

SAGE and its applications

Weiyuan Yu

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Abstract

Serial analysis of gene expression,or SAGE,is a high-throughput technique for the study of gene expres-sion pattern.SAGE allows for a quantitative and simultaneous analysis of a large number of transcripts in any par-ticular cells or tissues.In this article,the principle and the experimental procedure of SAGE,together with some modifications of SAGE method during the past few years are reviewed,then several research using SAGE method and some SAGE databases available on the Internet are introduced.

About this research paper

What this paper is about

Serial analysis of gene expression,or SAGE,is a high-throughput technique for the study of gene expres-sion pattern.SAGE allows for a quantitative and simultaneous analysis of a large number of transcripts in any par-ticular cells or tissues.In this article,the principle and the experimental procedure of SAGE,together with some modifications of SAGE method during the past few years are reviewed,then several research using SAGE method and some SAGE databases available on the Internet are introduced.

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Available abstract

Serial analysis of gene expression,or SAGE,is a high-throughput technique for the study of gene expres-sion pattern.SAGE allows for a quantitative and simultaneous analysis of a large number of transcripts in any par-ticular cells or tissues.In this article,the principle and the experimental procedure of SAGE,together with some modifications of SAGE method during the past few years are reviewed,then several research using SAGE method and some SAGE databases available on the Internet are introduced.

Key concepts: SAGE, Serial analysis of gene expression, Biology, Computational biology, Gene expression, Gene, Computer science, Genetics

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