Pharmacokinetics studied on berberine hydrochloride acted by rhizoma coptidis-fructus evodiae herbal couple in acute gastric ulcer rats

Objective To explore the pathological effect on berberine hydrochloride based on the pharmacokinetic research of berberine hydrochloride acted by rhizoma coptis-fructus evodia herbal couple in normal rats and acute gastric ulcer rats respectively.Methods Rats in each group were orally administered with rhizoma coptis-fructus evodia herbal couple(6:1),and then the in vivo plasma concentrations of berberine hydrochloride were determined by high performance liquid chromatographic(HPLC) method.Chromatogram column: Diamonsil C18(150mm×4.6mm,5μm);the mobile phase: Acetonitrile-methanol-0.05mol/L potassium dihydrogen phosphate solution(23:12:65);the flow rate: 1.0ml/min;the UV detector was operated at 345 nm and the column temperature was controlled at 30℃.Results The main pharmacokinetic parameters of berberine hydrochloride in the two groups were as follow.Pharmacokinetics parameters of the normal group of berberine hydrochloride: Cmax=(0.064±0.01)μg/ml;Tmax=(0.42±0.13)h;t1/2=(5.96±1.16)h;AUC0-t=(0.42±0.08)μg/(ml·h);AUC0-∞=(0.45± 0.09)μg/(ml·h);Pharmacokinetics parameters of the acute gastric ulcer group of berberine hydrochloride: Cmax=(0.069±0.01)μg/ml;Tmax=(0.54±0.11)h;t1/2=(5.11±1.78)h;AUC0-t=(0.60±0.17)μg/(ml·h);AUC0-∞=(0.63±0.18)μg/(ml·h).Conclusion Rhizoma coptis-Fructus evodia herbal couple can slow the absorption but increase the dose and retention time in plasma of berberine hydrochloride in the acute gastric ulcer rats caused by alcohol.