Pathogenicity of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against larvae of Batocera davidis

Batocera davidis Deyrolle is a new pest of poplar in Fujian.In order to screen entomopathogenic fungi strains with high virulence against B.davidis larvae,and bioassay evaluation of B.bassiana and M.anisopliae against B.davidis larvae was carried out through dipping in spore suspension.The results showed that the cumulative corrected mortality of B.davidis larvae was gradually increased with time after inoculated with B.bassiana and M.anisopliae.The corrected mortality of B.davidis larvae on the 14th day after inoculated with Ma1291-2 and Ma1775 were 91.69% and 86.50%,respectively,and the mortality was 71.05% on the 14th day after inoculated with BbDp-06.The muscardine cadaver rate of larvae inoculated with M.anisopliae was significantly higher than that inoculated with B.bassiana.While LT50 value of the two M.anisopliae strains,Ma1291-2 and Ma1775 were 5.73 and 5.39 days,respectively,and LT50 value of B.bassiana BbDp-06 was 8.02 days.LC50 value of two strains,Ma1291-2 and BbDp-06 were 3.82×104 spore · mL-1 and 3.88×104 spore · mL-1,respectively.The results of bioassay showed that Ma1291-2 and Ma1775 strains had higher virulence to B.davidis larvae than BbDp-06 strain.A separate field bioassay suggested that the 2 strains,Ma1291-2 and Ma1775 could be used to control B.davidis larvae.