Study of inducing H22 cell apoptosis action of triptolide

Objective It is to study the inducing H22 cell apoptosis action of triptolide.Methods The inhibition ratio of triptolide on H22 tumor cell was determined with MTT method.The inducing H22 cell apoptosis action of triptolide was observed and detected with cell morphology and DNA gel electrophoresis analysis.Results The inhibition ratio of 80 μg/L triptolide was 41.6%.It was positively correlated with concentration and the IC50 was 85.79 μg/L.The electron microscope result manifested that the concentration of triptolide more than 40 μg/L could obviously induced H22 cell showing apoptosis characteristics.DNA electrophoresis showed obvious ladder shape strap.Conclusion Triptolide can induce H22 cell apoptosis,it shows that triptolide has certain antihepatocarcinoma effect.