Analysis of Genetic Diversity and Relationship of Glycine soja in Northeast China

The genetic diversity of 52 northeast wild soybeans were analyzed by 60 pairs of SSR primers.The results showed that: 408 alleles were detected by 40 pairs of SSR markers,with an average of 12.5 alleles for each locus.Shannon-Weaver diversity indexes varied from 1.2203 to 2.6392 with averaged 2.0560,wild soybean has a rich genetic variation in northeast China;wild soybean in 41~43°N×124~126°E area have the richest accessions and genetic diversity,thus the Liaohe plain might be the newborn genetic diversity centre.Wild soybean in 39~41 °N × 120~122 °E region have higher genetic diversity index,but fewer resources were collected,thus the Liaodong hilly area might be the secondary genetic diversity centre of the northeast China.It proved that the formation of two uneven wild soybean centers of diversity in northeast China.Based on SSR data,52 wild soybeans were clustered into six categories,the results of clustering was related with the geographical distribution of wild soybeans.