Differences of neurite outgrowth induced by rho kinase inhibitors between in PC12 cell line and PC12 Adh cell line

PC12 cell line is one of the basic tool cell lines used in neural differentiation research.The cell line is able to grow neurite by exposure to rho kinase(ROCK) inhibitors.Recently,American Type Culture Collection(ATCC) provided PC12 cell line and PC12 Adh cell line.The main aim of the study was to clarify the differences of the two cell lines in neurite outgrowth induced by ROCK inhibitors.PC12 cell line and PC12 Adh cell line(from ATCC) were treated with nerve growth factor(NGF,1000ng/mL) or ROCK inhibitors(Y27632 of 33 μmol/L and fasudil of 33 μmol/L) for 1 —4 days.NGF was able to promote neurite outgrowth both in PC12 cell line and PC12 Adh cell line,while ROCK inhibitors was able to do so only in PC12 Adh cell line.These results suggested that PC12 Adh cell line is more suitable for ROCK inhibitors study in neural differentiation.