The effect of Ro25-6981 on the neurogenesis in adult rat hippocampus following transient forebrain ischemia/reperfusion

Objective To investigate the effect of the selective antagonist Ro25-6981 of NMDA receptor subunit 2B(NR2B) on the neural recovery in adult rat hippocampus after transient forebrain ischemia/reperfusion(I/R).Methods The male SD rats were randomized into three groups: the experiment groups(Ⅰand Ⅱ) and the control group.The rats in the experiment groups and the control group were subjected to 15 min of 4-artery occlusion(4AO) to establish the animal model of rat brain ischemia(15 min) and reperfusion.The rats in the experiment groups were intraperitoneally administered with Ro25-6981 at doses of 0.3 mg/kg and 0.6 mg/kg,respectively,while the same volume of NS was injected into the rats in the control group.Immunohistochemistry was employed to demonstrate and to compare the rate of BrdU positive cells in CA1 of hippocampus in different groups at 1 d,3 d and 7 d.Results The control group: BrdU positive cells peaked at I/R 3 d and decreased until I/R 7 d in the CA1 region.The experiment groups: The numbers of BrdU-positive cells significantly decreased as compared to that in the control group.Conclusion Transient forebrain ischemia/reperfusion could induce neurogenesis in the CA1 region,while this kind of neurogenesis could be inhibited by Ro25-6981.