2012•Journal of Hubei University of MedicineRequires access
Expression of RhoA in Spinal Cord of Rats with Neuropathic Pain
Huai-jie Yang
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Abstract
Objective To observe the expression of RhoA in spinal cord of rats with neuropathic pain and its possible role.Methods Thirty adult male Wistar rats weighing 180~220 g were randomly divided into sham group and neuropathic pain group(n=15).The neuropathic pain model were prepared in rats by ligating L5 spinal nerve.The L5 spinal nerve of rats in sham group were just exposed without ligation.Five rats were sacrificed in each group at day 3,7 and 14 post-operation.The expression of RhoA in spinal cord was determined by immunohistochemistry and Western blot.Results Compared with sham group,astrocyte and microglia in spinal cord were obviously activated and highly expressed RhoA from day 3 to day 14 by ligation in rats of neuropathic pain group.Conclusion The expression of RhoA in astrocyte and microglia is up-regulated in rats with L5 spinal nerve ligation,which may play an important role in the development of neuropathic pain.