Establishment of Mice Liver Injury Model by Isoniazid Combined with Rifampacin

OBJECTIVE:To provide reference for the establishment of mice liver injury model by isoniazid combined with rifampacin.METHODS:32 mice were randomly divided into control group(normal saline) and model group(isoniazid and rifampacin,each 75 mg·kg·d-1).Mice were given medicine via i.g.gtt.once every day at dose of 20 mL·kg·d-1.Mice were sacrificed on the 7th day and the 14th day.And then aminotransferase(ALT),aspartate aminotransferase(AST) activity of serum were assayed.Pathological changes of liver in experimental mice were observed.Contents of malondialdehyde(MDA) and super-superoxide dismutase(SOD) activity were assayed.RESULTS:Compared with control group,ALT and AST of serum increased significantly in model group on the 7th day and the 14th day.MDA content increased significantly while SOD activity was significantly reduced in model group on the 14th day.Compared the 7th day,AST of serum increased significantly in model group on the 14th day(P0.05 or P0.01).Pathological changes showed that on the 7th day liver cells arranged in cord disorders and cell volume increased.There were a small amount of liver cell necrosis and inflammatory cell infiltration in model group.On the 14th day,liver cells arranged in cord disorder,liver cells necrosis was more severe with significant inflammatory cells infiltration.No significant liver damage was found in control group.CONCLUSION:The method can establish experimental model of liver injury in mice successfully.